gyrA polymorphism in Campylobacter jejuni: detection of gyrA mutations in 162 C. jejuni isolates by single-strand conformation polymorphism and DNA sequencing.

نویسندگان

  • Antti Hakanen
  • Jari Jalava
  • Pirkko Kotilainen
  • Hannele Jousimies-Somer
  • Anja Siitonen
  • Pentti Huovinen
چکیده

Mutations in the quinolone resistance-determining region of the gyrA gene from 138 ciprofloxacin-resistant (MIC, > or =4 microg/ml) and 24 ciprofloxacin-susceptible (MIC, < or =1 microg/ml) clinical Campylobacter jejuni isolates were subjected to single-strand conformation polymorphism analysis and sequencing. All of the isolates could be assigned to three genotypic clusters based on silent mutations. All resistant isolates had a point mutation at codon 86.

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Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis.

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 46 8  شماره 

صفحات  -

تاریخ انتشار 2002